BIOCHEMICAL PARAMETERS, PROGESTERONE CONCENTRATION and CONCEPTION RATES in POSTPARTUM ANESTRUS BUFFALOES TREATED WITH MODIFIED OVSYNCH and MODIFIED COSYNCH PROTOCOLS

The present study was planned to evaluate the biochemical parameters, progesterone concentration and conception rates in postpartum anestrus buffaloes treated with modified Ovsynch and Cosynch protocols.A total of 120 postpartum buffaloes reared under field conditions in Jabalpur (M.P.) were surveyed during the study. The buffaloes with the history of not showing signs of oestrus for 60 days or more postpartum were considered to be suffering from postpartum anestrus. These buffaloes were per rectally examined twice at 10 days apart to confirm ovarian activity.Twenty four postpartum anestrus buffaloes were randomly selected from the buffaloes found positive for anestrus. These animals were again randomly divided into 4 groups (n=6 animal/group). Group I, II and III animals were treated with CIDR plus Ovsynch, CIDR plus Cosynch and eCG plus Cosynch protocols, respectively, while the control group (Group IV) animals were not given any treatment. All treatment group animals were bred by fixed time artificial insemination while control animals were bred on observed oestrus by artificial insemination. Serum progesterone (ng/ml) levels were estimated by ELISA and biochemical analysis (like Calcium, Phosphorus, Magnesium, Total protein and Total cholesterol) were done by semi-automatic analyzer on 0 day and day 21 post artificial insemination. Pregnancy diagnosis was done by per rectal examination 60 days post insemination. Mean serum total cholesterol concentration on day 21 post insemination varied significantly between group I and III. Mean serum progesterone concentration decreased significantly (p0.05) in group II on day 21 post insemination as compared to day 0. The conception rates in group I, II, III and IV were 50.00, 33.33, 66.67 and 33.33 per cent, respectively. It was concluded that eCG+ Cosynch protocol was better as compared to CIDR+Cosynch and CIDR + Ovsynch in postpartum anestrus buffaloes.