Research article

DETECTION AND DETERMINATION OF 4-METHYLIMIDAZOLE IN FOOD SAMPLES CONTAINING CARAMEL PIGMENT

Dhay abood Jaleel 1 and Alyaa S. AL- Hafud 2

Online First: February 22, 2023


This study was conducted to demonstrate the negative effect of 4-methylimidazole on some health parameters that included liver and kidney enzymes in rats. Forty male rats, aged between 3-4 months and weighing 25 ±, were used in this experiment, divided into 4 groups as follows: Which included (10) animals in each group, the control group was left without any treatment. They were given tap water and diet, and group G1 was dosed with the substance at a concentration of 20 mg/kg, and group G2 was dosed with the substance at a concentration of 40 mg/kg. Group G3 was dosed with the substance at a concentration of 80 mg/kg, for a period of (35) days. The results showed that there was a significant increase in the level of creatinine in the group treated with a concentration of 80 mg / kg of body weight compared with the control group, while the results did not show a significant difference between the control group and the two groups treated with a concentration of 20 and 40 mg / kg. The results also showed a significant increase in the blood urea level in the groups treated with concentrations (20, 40 and 80) mg/kg, and the results also showed a significant increase in (ALP, ALT, AST) enzymes in the groups treated with concentrations (20, 40, 80) mg. /kg,The results showed that there was a significant decrease in the final body weight in the groups treated with concentrations of (40, 80) mg / kg, compared with the initial weight of both concentrations, respectively. While the results showed a significant increase in the final body weight of the control group compared with the initial weight. While the results did not show a significant difference in the initial and final body weight of the treated group with a concentration of (20) mg / kg of body weight. In the histological study of the histological sections, there were changes in hepatitis with infiltration and focal necrosis in the groups (G1, G2, G3) compared with the control group.

Keywords

caramel pigment, 4-methylimidazole, creatinine.