GENETIC VARIATION OF ACINETOBACTER BAUMANII ISOLATED FROM DIFFERENT SOURCES

Samples were collected from Kirkuk governorate during the period between December 2019 to March 2020. 8 bacterial isolates were collected and diagnosed from clinical infections (wounds, burns, urinary tract infection, Sputum) and environmental sources (operating room bed, split in operating room, wall in a preterm room). RAPD reactions were carried out using the Accupower PCR pre mix kit supplied by the Korean company Bionneer, according to the instructions attached to it, and each tube contains the basic components of the polymerase chain reaction, which include one unit of dNTPs, Tris-HCL 10Mm PH= 9, MgCL2 5.1, 30Mmkcl, and Taq DNA Polymerase enzyme, and samples were prepared for migration by PCR. Random primers whose sequences range from 5 to 3 were used, as they included the primer OPA-01 with the CAGGCCCTTC nucleotide sequence and the OPA-06 primer with the GGTCCCCTGAC nucleotide sequence. The initiator OPA-01 showed the location of the bands, and they were all different, their molecular sizes ranged between (50-2000bp), the number of bands reached (53) bands, and ranged between (4-8) bands for the sample in the results of this primer. Sample (3) was characterized by a unique band with a molecular size (50bp). Sample (6) was characterized by the appearance of a unique band of molecular size (200bp). Sample (5) was characterized by the presence of an absent band of molecular size (2000bp). Sample 1) was characterized by the presence of an absent band of molecular size (1000bp). The sample (4) was characterized by the presence of an absent packet with a molecular size of (900bp) and the contrast ratio of this primer was 100%, and its efficiency was 7.11 and its discriminatory ability 3. The results of the OPA-06 primer showed the location of the packets, and they were all different, their molecular sizes ranged between (200-2000bp) amounting to a number of Packages (47) ranged between (3-7) per sample, in the results of this primer. Sample (3) was characterized by the presence of a unique bundle of molecular size (1000bp). The sample (5) was characterized by the presence of a unique bundle of molecular size, respectively (2000 bp, 400, 250, 200). The sample (6) was characterized by the presence of a unique band with a molecular size of (300bp). And sample (7) has a unique band of molecular size (225bp). Sample (2) was characterized by the presence of an absent band with a molecular size of (600bp), and the contrast ratio was 100%, and its efficiency was 3.10 and its discriminatory ability was 9.10.