Research article

MEDIA FACTOR USES FOR ISOLATION TRANSFORMATION AGROBACTERIUM STRAIN LBA4404 FROM OTHER BACTERIA

Jimma K. Mohmmad1 Ismail H. Aziz 1 Harith K. Buniya2

Online First: March 14, 2023


Growing Agrobacterium for commercial generation of recombinant proteins via transient expression has not been thoroughly studied in the literature. The effects of media composition on Agrobacterium growth for transient expression were examined in this work. Lysogeny broth (LB) media (also known as Luria-Bertani media or Luria Broth) 10 and yeast extract-peptone (YEP) media are extensively utilized in studies for Agrobacterium growth. Benthamiana leaves infiltrated with Agrobacterium in chemically specified AB media outperformed leaves infiltrated with Agrobacterium in LB media in terms of transient expression level and post-incubation leaf health

Keywords

Agrobacterium, (LB) media, (YEP) media