Research article

ISOLATION AND MOLECULAR IDENTIFICATION STUDY OF ESCHERICHIA COLI FROM PATIENTS WITH URINARY TRACT INFECTIONS (UTIS) AND STUDY OF ANTIBIOTIC RESISTANCE AND ANTIBACTERIAL EFFICACY OF FLAXSEED OIL IN ANBAR, IRAQ

1Tariq Talal Khalaf,2Ashwak B. AL-Hashimy, 3 Yasir Merie Nayyef.

Online First: March 14, 2023


Two hundred and ten mid-stream urine of were taken from UTI patients attending Al-Falluja Teaching Hospital and Al-Ramadi Teaching Hospital for Maternity and Children. The samples were cultured on MaCconkey agar, eosin methylene blue agar, blood agar biochemically tested were done to confirm the diagnosis. All bacterial isolates assured identified by VITEK® 2 Compact system and 16srRNA. To sum up 60 (28,5%) out of 210 (100%) UTI samples showed growth of E.coli whereas, 43 (20.5%) out of 210 (100%) UTI samples showed no growth of bacteria. Also, 107 (50.9 %) out of 210 (100%) UTI samples showed growth of different types of bacteria such as staphylococci, streptococcus, proteus, K.pneumonia, and Acinetobacter. The antimicrobial susceptibility test performed by VITEK® 2 Compact system and disk diffusion method demonstrated that the isolates showed a high rate of resistance to the third generation of cephalosporins. The presence of antibiotic-resistant genes was determined by polymerase chain reaction (PCR) using specific primer for (16srRNA, TEM) genes. The antimicrobial activity of flax (Linum usitatissimum) seed oil has been studied and showed significant antimicrobial activity against all tested bacterial isolates of E.coli. which indicates that the substance used under study had a significant effect on reducing the gene expression of the antibiotic resistance gene.

Keywords

urinary tract infection (UTI), Escherichia coli, blaTEM, flaxseed oil