Research article

FORMULATION OF ANTI-ACNE CREAM PREPARATIONS AND ANTI-BACTERIAL ACTIVITY OF ROSELLA FLOWER ETHANOL EXTRACT (HIBISCUS SABDARIFFA L.) AGAINST PROPIONIBACTERIUM ACNE AND STAPHYLOCOCCUS EPIDERMIDIS

Devannie Alberta Willim1, Jesslyn Tiofanny1, Ali Napiah Nasution*1, Evelyn Angie1

Online First: March 24, 2023


Acne vulgaris (acne) is a skin disease caused by chronic inflammation with a complex pathogenesis involving the sebaceous glands, follicular hyperkeratinization, excessive bacterial colonization, the body's immune reaction, and inflammatory triggers for acne, including genetics, hormonal activity in the menstrual cycle, stress, sebaceous gland activity hyperactivity, hygiene, food, and use of cosmetics. One of the factors that cause acne is bacteria. Propionibacterium acnes and Staphylococcus epidermidis are the bacteria causing acne. Antibiotics are quite effective in dealing with acne, but the use of antibiotics as the main option for treating acne must be reviewed to limit the development of bacterial resistance to antibiotics. One of the traditional medicines that are now widely used in society is Rosella flower (Hibiscus sabdariffa L.). The aim of the research was to determine the formulation of rosella flower ethanol extract cream (Hibiscus sabdariffa L.) which has anti-acne activity against Propionibacterium acne and Staphylococcus epidermidis bacteria. Therefore, the inhibition test of rosella flower ethanol extract was carried out using the agar well diffusion method using cream without extract as a negative control and tetracycline well as a positive control. The inhibition test of cream of the rosella flower ethanol extract was carried out using concentrations of 5%, 10% and 15%. The results of this study showed that the cream of the ethanol extract of rosella flowers (hibiscus sabdariffa l.) at each concentration could inhibit Staphylococcus epidermidis and Propionibacterium acnes due to the presence of an inhibition zone or clear zone around the wells.

Keywords

Acne Vulgaris, Propionibakterium acne, Staphylococcus epidermidis and Extract of Rosella Flower (Hibiscus sabdariffa L.)