Research article

HISTOPATHOLOGICAL EVALUATION CHANGES OF STAPHYLOCOCCUS AUREUS SONICATED ANTIGEN AGAINST SALMONELLA TYPHIMURIUM INFECTION IN ALBINO RABBITS

Zyad M. M. Abu Aleiss, Ikram, A. A. AL-Sammarraae

Online First: January 20, 2023


This research aimed to evaluate the histopathological changes of sonicated antigens of Salmonella typhimurium and Staphylococcus aureus in the infected Albino rabbits by S. typhimurium. Twenty four Albino rabbits were randomly divided into three groups (eight animals/group). The first group was immunized by killed whole cell sonicated Ag of Salmonella typhimurium (KWCSAg-S.T) 1mg/ ml/animal subcutaneously. The second group was immunized with mixed KWCSAg-S.T 1mg/ml/animal and killed whole cell sonicated Ag of Staphylococcus aureus (KWCSAg-S.A) 1mg/ml/animal subcutaneously. The third group as a positive control was injected phosphate buffer saline (PBS) 1ml/animal subcutaneously. After 19 days, all groups of rabbits were given an encounter infective dose 9x108 CFU/ml/rabbit of S. typhimurium orally. All animals were sacrificed after seven days .Kidney and spleen of 1st and 2nd groups showed cellular infiltrations of mononuclear cells, and mild lesions in intestines compared to positive control group that showed a severe lesions in kidney, spleen and intestine. In conclusion, a foundation of resistance of immunization rabbits with sonicated antigens of S. typhimurium and S. aureus compared to non-immunized (positive control group) that suffer from severe histopathological lesions in the some internal organs.

Keywords

Salmonella, typhimurium, Staphylococcus, aureus, rabbit, histopathology.