Research article

DETECTION OF THE SUSCEPTIBILITY TEST, BIOFILM FORMATION IN ACINETOBACTER BAUMANNII ISOLATED FROM DIFFERENT SOURCES IN KIRKUK CITY

Saman kareem Amin Albarznjee1, Suha Maher Abed1, Adnan Fadhel Alazawy1

Online First: January 30, 2023


This research presents the isolation of Acinetobacter baumannii from different clinical sources such as burns, wounds, sputum and blood. A total of 10 isolates from the studied bacteria were collected and identified using microscopic examinations, culture, biochemical tests, and final diagnosis using the API 20 E system, as well as the use of polymerase chain reaction to detect the presence of the 16srRNA gene to confirm and identify Acinetobacter spp (molecular methods) by PCR. The bacteria showed growth at different temperatures until at 44°C. Antibiotic sensitivity test for 12 antibiotics was carried out using the Kirby-Bauer publication method. The results showed that the isolates were highly resistant to antibiotics, so that the isolates were 100% resistant to Ampicillin, Amoxicillin, Piperacillin, chloramphenicol, cefotaxime, Amoxicillin-Clavulanc acid, Amikacin And resistance to Tetracycline antibiotic by 90% and resistance to antibiotics Ceftazidime, Lefofioxacin, Trimethoprim-Sulphamethoxazole by 80%, while the most effective antibiotic against isolates was imipenem, which gave sensitivity by only 40%. Some virulence factors were examined, such as the ability of A.baumannii bacteria to form biofilms using the microplate method. The percentage of biofilm-forming isolates was 8/10 (80%), and the isolates were divided into four groups, 2 (20%) non-cell membrane-forming, 1 (10%) ) Biofilm formation was strong, 5 (50%) was moderate, 2 (20%) was weak. The DNA of A. baumannii isolates was extracted, and the csuE gene, which has a role in the formation of biofilms, was detected by PCR. Gel electrophoresis showed that the csuE molecular weight was 168 base pairs, and the percentage of isolates carrying the csuE gene was 100%.

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