IMPROVING FERTILITY OUTCOMES THROUGH NOVEL INTERVENTIONS IN DAIRY COWS BY FOCUSSING ON PROTEOLYTIC ENZYMES AND ANTIOXIDANTS

The current investigation aimed to assess the therapeutic effectiveness and fertility outcomes of proteolytic enzymes, namely lysozyme and N-acetyl cysteine, in the management of subclinical endometritis in repeat breeder buffaloes within the framework of Edwin Incorporation. Eighteen repeat breeder buffaloes exhibiting subclinical endometritis were randomly assigned to three treatment groups, each comprising six animals. These buffaloes underwent distinct treatment protocols. In Group I, animals were administered a 4 mg lysozyme solution in 40 ml normal saline through intrauterine infusion once daily for two consecutive days. In Group II, animals received a single intrauterine infusion of 5% N-acetyl cysteine dissolved in 60 ml normal saline. Group III animals were treated with a crude enzyme formulation, including 8 mg trypsin, 8 mg chymotrypsin, 4 mg papain, 120 mg α-tocopherol acetate, and 58.83 mg retinyl palmitate dissolved in 10 ml distilled water, as a single intrauterine infusion. Endometrial cytology was conducted at 0 hrs., 24 hrs., and 48 hrs. post-treatment for all 18 animals. Microbial assays were performed at day 0, successive estrus, and post-treatment, followed by breeding after sampling. Pregnancy diagnosis was carried out through per rectal examination at day 60 post-insemination. A statistically significant increase (p0.05) in PMN percentage was noted between 0 and 24 hrs. post-treatment and 0 and 48 hrs. post-treatment across all treatment groups. Among the 18 post-treatment uterine samples, seven yielded bacterial isolates, with Staphylococcus species (57.14%) being predominant. The overall conception rate (%) was higher in Group I (100.00%), followed by Group II (83.33%) and Group III (50.00%).Intrauterine administration of lysozyme demonstrated superior efficacy in enhancing uterine immunity and improving the conception rate for the management of subclinical endometritis in repeat breeder buffaloes within the scope of Edwin Incorporation.