Research article

ENHANCING THE BACTERIAL SYSTEM IN THE INTESTINES OF THE GRASS CARP CTENOPHARYNGODON IDELLA BY FEEDING IT ON DIETS OF DIFFERENT PROPORTIONS OF COW RUMEN CONTENTS ENRICHED WITH THEPAX YEAST

Yaas Khodair Atheeb Al-Rikabi and Prof. Assist. Dr. Taghreed Sadiq Mohson Al-Obaidy

Online First: March 14, 2023


The study was conducted in the Fish Laboratory, College of Agricultural Engineering Sciences / University of Baghdad, with the aim of using cow rumen contents enriched with prebiotic as a ration for grass carp fish. 15 fingerlings, the fish were fed on four types of diets equal in protein after crushing and grinding the materials and adding the rumen contents at rates of 10%,15%,20%, supplemented with prebiotic 2 g/kg except for the control diet free of addition. The fish were weighed every 14 days. The grass carp fish were dissected, cut with scissors along the side line of the fish, and the intestines were extracted. We took the middle and last part of the intestine, and one gram of the fish intestines was transplanted into two activated media in petri dishes after sterilization, left in the incubator for 48 hours. Colonies for Bacillus nervosa were counted. The results of the research indicated that the second treatment was significantly (p<0.01) superior to all treatments in terms of body weight rates, weight gain, relative and qualitative weight rates, food conversion ratio, food conversion efficiency and apparent digestion coefficient, followed by the third treatment, then the fourth treatment and for all durations of the experiment. The results showed that the second treatment was significantly superior to the rest of the treatments in this trait for the preparation of Bacillus subtilis isolated from the intestines of grass carp fish.

Keywords

prebiotics culture, grass carp. rumen content