PREVALENCE OF BIOFILM FORMATION IN BACTERIAL PATHOGENS IN VAGINAL WOMEN INFECTIONS
Huda J. Rashed 1, Jasim H. Makhrmash 2, Abeer H. Maslat 3
Background and aims: Bacterial vaginosis (BV) is a vaginal microbiome dysbiosis that is reported to be the most common gynecological condition in women of reproductive age. Methods: A total 120 swab samples were collected from patients with age 18 –50 years, during the period from 7th November, 2021 to 28th February, 2022 that admitted to Al-Zahra Teaching Hospital, Al Kut Hospital, , and private clinics in Wasit province. A total 70 bacterial isolates were detected by culture characterization on the different media, biochemical investigation, analytical profile index 20 and Vitek2 automated system and investigation about biofilm production using microtitre plate (MTP). Results: There were 70 bacterial-isolated samples. Results of this study has been showed that the high rate of bacterial biofilm formation was in recorded as 17.1%, 14.3% and 12.9% in Escherichia coli, klebsiella pneumoniae and staphylococcus epidermidis respectively, as they form strong biofilm.biofilm formation was more prevalent in gram-negative bacteria (73%), Klebsiella pneumoniae (37.41%), Escherichia coli (28.78%) Staphylococcus spp. (23.57%) was the only gram-positive isolate formed strong biofilm. Conclusion: In Wasit province, there is a development in bacterial production of biofilm. This may have a harmful impact on Iraqi women and should be seen as a concerning health situation.