Research article

IN VITRO: INVESTIGATION THE EFFECT OF BACTERIA ISOLATED FROM BACTEREMIA PATIENTS ON HEMATOLOGICAL PARAMETERS AND ITS NUMBER IN BLOOD

Abbas Hameed Al-Wandawy1, Luma Abdulhady Zwain2, Dalia Maher Khidher3

Online First: January 15, 2023


The bloodstream in a healthy human is generally a sterile environment and the presence of bacteria in it is a indicate of bacteremia, most body fluids in healthy people do not contain bacteria such as blood so the presence of infection can affect the patient's life. 313 blood samples were collected from bacteremia pationts, including 146 (30 patient and 116 samples of out patients) from Azadi Teaching Hospital, (36) from the dialysis Unit at Kirkuk General Hospital , 126 samples (42 samples of patients and 84 samples of out patients) from the Children's Hospital, and (5) samples from the Women’s and Obstetrics Hospital in Kirkuk province, for the period from the twenty-fourth of January 2022 to the tenth of September 2022. Blood samples were cultured in the Brain heart infusion broth and incubated at 37 ° C for 24 hours, then cultured on the MacConkey agar and Blood agar and the results showed that 29(58%) isolates were gram positive gram bacteria and 21 (42%) gram negative. Twenty-eight isolates of the gram positive belonged to negative coagulase Staphylococcus, and most of them were 37.93% S.hominis bacteria, while negative gram bacteria was mostly the Pseudomonas aeruginos by 52.38% ,this isolated bacteria studied on the hematological parameters , by use the EDTA tubes containing blood (healthy) ,than inoculated with S.hominis and P.aeruginosa. It was noted from the results that the hematological parameters (WBC, MID%, NEUT%, LYM#, NEUT#, RBC, HGB, HCT, MCV, MCH, MCHC, RDW-SD, RDW-CV, MPV, PDW, PCT, P-LCR) were not affected at a growth density of 1.5×108 and 3×108 for S.hominis and P.aeruginosa bacteria at zero time, 4 hours and 24 hours except for the PLT and the LYM% criterion was different according to the incubation period. Then the effect of blood on the bacteria S.hominis and P. aeruginosa was studied and the results showed a decrease in the number of bacteria at (2, 4 ,24) hours of incubation with more decrease after incubation 24 hours, and the decrease in the number of gram positive bacteria was more than the gram negative bacteria at (2, 4) hours of incubation.

Keywords

Bacteremia, CBC, Bacteria, Blood.