Research article

THE EFFECT OF HESPERIDIN TO KIDNEY TYPE L- GLUTAMINASE EXPRESSION AND MOLECULAR DOCKING STUDY

Ali G. Al-Dulimi a, ziyad k. Radeef a, Shahrazad A. khalaf b

Online First: January 15, 2023


Different plant sections from (Laurus nobilis, Malus domestica, and Citrus limon) were extracted using 80% methanol. All plants were then evaluated phytochemically for total flavonoids, with different concentrations of 952.77173.47, 980.55673.57, and 341.6683.33 mg/ml, respectively. Additionally, all plant extracts outperformed trolox in terms of their ability to reduce. Additionally, plants' radical scavenging abilities are stronger than vitamin C. (positive control). Some active plant chemicals, including hesperidin, quercetin-3-rhamnoside, and Q-3-O-B-glucopyranoside, underwent molecular docking. Due to its chemical structure, hesperidin had a stronger binding affinity with the active site of human kidenny type l-glutaminase (KGA) with a binding energy of (-7.09 kcal/mol) than the reference (crystal ligand), which had a binding energy of (-6.96 kcal/mol). Hesperidin peak is shown by HPLC analysis of Citrus limon methanolic extract at retention time 14.80 ppm. Purified hesperidin provided an inhibition of 65.33% for standard KGA in an in vitro inhibitory experiment when compared to CB-839 positive control. Using RT PCR, the GLS1 gene expression levels in the AMJ13 cell line were assessed after treatment with hesperidin and CB-839. The results revealed that hesperidin suppressed GLS1 gene expression in the cancer cell line while CB-839 increased GLS1 gene expression.

Keywords

Hesperidin; molecular docking; KGA; breast cancer; GLS1gene expression