EFFECT OF GROWTH REGULATORS (NAA AND BA) ON CALLUS FROM MORINGA OLEIFERA L. IN VITRO.

Effect of growth regulators (BA) - (IAA-BA) - (NAA) at concentrations (0-0.5-1.0-1.5-2) mg/L of callus produced from (2,4-D-BA) produced from (Moringa oleifera L. ) in vitro. Where there was a significant effect of the duration of sterilization in reducing the percentage of pollution, which reached the lowest value of 22.50% at the time period of 20 minutes. As for the concentration, we note that the 20% concentration gave the lowest contamination rate, which amounted to 58.50%, which was significantly excelled on all other concentrations. As for the interaction between the concentrations and the time of treatment, 20 with concentration 20, as the lowest contamination rate reached 15%. Also, there was a significant effect of the interaction between the concentrations of auxin and cytokinin in the effect on the fresh weight of the callus. The treatment (4) of 2,4-D at a concentration of (1.5) mg/L interaction with BA at a concentration of (2) mg/l-1 in giving The highest callus fresh weight was 1.45 mg and the lowest was when the control treatment was 0 from 2,4-D × BA It reached (1.04) mg. Also, the interaction between the concentrations of auxin and cytokinin was significant in affecting the fresh weight of the callus, and treatment (3) of IAA with a concentration of (1.0) mg/L interaction with BA at a concentration of (0.5) mg/L-1 excelled in giving the highest fresh weight of the callus. It reached (830 mg), and the lowest treatment is when the control treatment is 0 from NAA x BA it reached 238 mg.