Research article

ON THE NATURE OF TRANSPOSITION OF CATEGORICAL FORMS OF THE RUSSIAN VERB

Popova E.I.

Online First: December 16, 2022


The article is devoted to the problem of the transposition of categorical forms of the Russian verb. The article describes the modal-semantic features of particular species-time values, which in the conditions of transposition acquire a figurative meaning. When describing cases of figurative use of time forms, the connection with the context is traced – the basis for the implementation of the connotative function: the lexical meaning of the verb, temporal qualifiers, syntactic factors, the correlation of the sentence with the situation are taken into account. !DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Above-ground biomass estimation in a Mediterranean sparse coppice oak forest using Sentinel-2 data
Research article

THE ADJUVANT POTENTIAL OF DEUTERIUM IN SUBUNIT VACCINE PREPARED FROM OUTER MEMBRANE PROTEIN OF PASTEURELLA MULTOCIDA

J. Jogi1*, A. Nayak1, A. Rai1, P. Shakya1, R. Gangil2, R. Sikrodia2, D. Chhabra2, R. Sharda2, K. Rawat2, and S. Bordoloi3

Online First: December 16, 2022


The present research work aimed to determine the adjuvant potential of deuterium in a subunit vaccine prepared from the outer membrane protein of Pasteurella multocida. P. multocida type B:2 causes Haemorrhagic Septicaemia (HS) in cattle and buffaloes. Humoral immunity gained through vaccination may play an important role in protection against HS. The Porin H (OmpH) is a major immunodominant outer membrane protein in the envelope of P. multocida. Deuterium / heavy water (D2O) might be utilised to increase the thermostability and the shelf life of the vaccines. Prepared subunit vaccine (recombinant OmpH) was tested for its immunogenicity by using deuterium as an adjuvant in adult albino mice. The animals were divided into five groups were immunized with recombinant OmpH with different concentrations of deuterium as 25, 50, 75 and 100%. The antibody titre and humoral immunity were assessed at a predetermined time interval starting from day 0 to 28 days. Antibody titres across groups showed a very significant difference (p≤0.01) in statistical analysis. Compared to the other four groups, the antibody titre in Group II (recombinant OmpH with 50% deuterium) was much higher. Similarly, Group II (recombinant OMPH with 50% deuterium) showed the maximum humoral immune response and was considered for the immunization trial of the thermostable property of deuterium. The overall study concluded deuterium is an excellent candidate for the enhancement of antibody titre and humoral response and it may play a pivotal role in the development of vaccines against the treatment of HS.

Keywords

Pasteurella multocida, Deuterium, Haemorrhagic Septicaemia, Porin H, Outer membrane protein

Keywords

transposition, transfer of language form, morphological transposition, figurative type of usage, direct and figurative meanings of specific forms, figurative - grammatical nature of context, temporal qualifiers, verb semantics, syntactic conditions.